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| Status |
Public on Dec 03, 2019 |
| Title |
Transcriptome signatures reveal candidate key genes in the whole blood of patients with lumbar disc prolapse |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The present study aimed to investigate differentially expressed genes in whole blood obtained from patients with lumbar disc prolapse and healthy volunteers. A total of 8 patients with lumbar disc prolapse and 8 healthy volunteers were recruited. An Agilent SurePrint G3 human gene expression microarray 8x60 K was used to perform the microarray analyses.
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| Overall design |
We recruited 8 patients with lumbar disc prolapse confirmed by magnetic resonance imaging, and 8 volunteers with no clinical evidence of low back pain or sciatica. Ten millilitres of fasting WB was collected from the left median cubital vein of each participant between 7:00 and 7:30 AM. All WB samples were incubated in a PAXgene Blood RNA tube (BD, USA) for less than 72 h at -20°C and then submitted to Shanghai Bohao Biotechnology Co., Ltd (Shanghai, China) for gene chip hybridization screening. The microarray experiments were performed using an Agilent SurePrint G3 human gene expression microarray 8 ╳ 60 K at Shanghai Biotechnology Co., Ltd (Shanghai, China) following the standard protocol of Agilent Technologies (CA, USA).
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| Contributor(s) |
Yi W |
| Citation(s) |
31777557, 33535986 |
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| Submission date |
Dec 21, 2018 |
| Last update date |
Feb 08, 2021 |
| Contact name |
Yi Wang |
| E-mail(s) |
yiwang1984@hotmail.com
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| Organization name |
Sichuan Provincial Orthopedic Hospital
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| Department |
Cervicodynia/Omalgia/Lumbago/Sciatica Department 2
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| Street address |
132 West First Section First Ring Road
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| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA511447 |
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