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Series GSE124266 Query DataSets for GSE124266
Status Public on Dec 21, 2019
Title TREM2 regulates microglial cholesterol metabolism upon chronic phagocytic challenge [bulk RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary TREM2 is a microglial-specific gene implicated in late-onset Alzheimer's Disease (AD). Recent studies have identified that Trem2-deficient mice exhibit transcriptional changes in microglial gene expression that minimize the upregulation of lipid metabolism and lysosomal genes in AD disease models. We find that chronic phagocytic challenge from demyelination generates similiarly attenuated expression of lysosomal and lipid metabolism genes in microglia isolated from Trem2 knockout mouse brain.
 
Overall design Trem2 wildtype, heterozyogus knockout, and homozygous knockout mice were fed a control or 0.2% cuprizone diet for 5 weeks or 12 weeks to generate acute or chronic demyelination, respectively. Microglial and pooled non-microglial cell type gene expression was assessed using 3' tag sequencing of poly-adenylated RNA (QuantSeq, Lexogen, Vienna, Austria).
 
Contributor(s) Nugent AA, Sandmann T, Monroe KM, Di Paolo G
Citation(s) 31902528
Submission date Dec 21, 2018
Last update date Mar 22, 2020
Contact name Thomas Sandmann
E-mail(s) genomics@dnli.com, sandmann@dnli.com
Organization name Denali Therapeutics
Street address 161 Oyster Point Blvd
City South San Francisco
State/province California
ZIP/Postal code 94080
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (74)
GSM3526593 Microglia cells from Trem2 +/- animal 1040 after 12 weeks on cuprizone diet
GSM3526594 Non-microglia cells from Trem2 +/- animal 1040 after 12 weeks on cuprizone diet
GSM3526595 Microglia cells from Trem2 -/- animal 1104 after 5 weeks on control diet
This SubSeries is part of SuperSeries:
GSE130627 TREM2 regulates microglial cholesterol metabolism upon chronic phagocytic challenge
Relations
BioProject PRJNA511441
SRA SRP174208

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Supplementary file Size Download File type/resource
GSE124266_DST11.tab.gz 1.9 Mb (ftp)(http) TAB
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Processed data are available on Series record
Raw data are available in SRA
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