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| Status |
Public on Dec 21, 2018 |
| Title |
The Possible Role of Complete Loss of Myostatin in Limiting Excessive Proliferation of Muscle Cells (C2C12) via Activation of MicroRNAs (mRNA-seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Myostatin (MSTN) is a member of the TGF-β superfamily that negatively regulates skeletal muscle growth and differentiation. However, the mechanism by which complete MSTN deletion limits excessive proliferation of muscle cells remains unclear. In this study, we knocked out MSTN in mouse myoblast lines using a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) system and sequenced the mRNA and miRNA transcriptomes. Our study suggests that complete loss of MSTN may limit excessive cell proliferation via activation of miRNAs.
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| Overall design |
mRNA profiles of wild type (WT) and MSTN-knockout (MSTN-KO) were generated by deep sequencing, in triplicate, using Illumina HiSeq 2000
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| Contributor(s) |
Huang P, Yu H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 20, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Wenni You |
| E-mail(s) |
1170321581@qq.com
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| Organization name |
Jilin University
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| Street address |
No 5333 Xi`an road
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| City |
Changchun |
| ZIP/Postal code |
130062 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE124196 |
The Possible Role of Complete Loss of Myostatin in Limiting Excessive Proliferation of Muscle Cells (C2C12) via Activation of MicroRNAs |
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| Relations |
| BioProject |
PRJNA510980 |
| SRA |
SRP174054 |
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