|
| Status |
Public on Jul 10, 2019 |
| Title |
The pause-initiation limit restricts transcription activation in human cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Eukaryotic gene transcription is often controlled at the level of RNA polymerase II (Pol II) pausing in the promoter-proximal region. Pausing Pol II limits the frequency of transcription initiation (‘pause-initiation limit’), predicting that the pause duration must be decreased for transcriptional activation. To test this prediction, we conducted a genome-wide kinetic analysis of the heat shock response in human cells. We show that the pause-initiation limit restricts transcriptional activation at most genes. Gene activation generally requires the activity of the P-TEFb kinase CDK9, which decreases the duration of Pol II pausing and thereby enables an increase in the productive initiation frequency. The transcription of enhancer elements is generally not pause-limited and can be activated without CDK9 activity. Our results define the kinetics of Pol II transcriptional regulation in human cells at all gene classes during a natural transcription response.
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| |
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| Overall design |
Multi-omics in human cells (TT-seq, mNET-seq, RNA-seq)
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| |
|
| Contributor(s) |
Gressel S, Schwalb B, Cramer P |
| Citation(s) |
31399571 |
| |
| Submission date |
Dec 17, 2018 |
| Last update date |
Aug 21, 2019 |
| Contact name |
Björn Schwalb |
| E-mail(s) |
bschwal@gwdg.de
|
| Organization name |
MPI
|
| Street address |
Fassberg 11
|
| City |
Göttingen |
| ZIP/Postal code |
37077 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (26)
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|
| Relations |
| BioProject |
PRJNA510428 |
| SRA |
SRP173667 |
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