|
| Status |
Public on May 17, 2019 |
| Title |
RNA-Sequencing of ST2+ Treg cells in response to IL-33 stimulation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The effects of IL-33 on ST2+ Treg cells were not studied thouroughly. We FACS-sorted in vitro expanded ST2+ Treg cells from C57BL/6 Foxp3-IRES-mRFP (B6 FIR) mice. We next used RNA-seq techonology to define how recombinant IL-33 (rIL-33) may impact mouse Treg by to assessing the transcriptome of IL-33-stimulated ST2+ Treg cells compared to that of untreated ST2+ Treg cells. Our data revealed that ST2+ Treg stimulated with rIL-33 for 6 hours exhibited increased expression of Il10 and Il13 compared to unstimulated ST2+ Treg cells.
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| |
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| Overall design |
Examine of transcriptome of rIL-33-treated or untreated ST2+ Treg cells.
|
| |
|
| Contributor(s) |
Turnquist HR, Chandran UR, Chakka AB |
| Citation(s) |
30779711 |
| |
| Submission date |
Dec 17, 2018 |
| Last update date |
May 17, 2019 |
| Contact name |
Uma Chandran |
| E-mail(s) |
chandran@pitt.edu
|
| Phone |
412-648-9326
|
| Organization name |
University of Pittsburgh
|
| Department |
BioMedical Informatics
|
| Street address |
5150 Center Ave
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15232 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA510330 |
| SRA |
SRP173591 |
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