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Series GSE123734 Query DataSets for GSE123734
Status Public on Dec 12, 2020
Title Functional dissection of MIEN1 trans-regulation provides new evidence for colorectal cancer genome editing-based therapeutics
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary MIEN1 has been described as a key actor in colorectal cancer (CRC) biogenesis. We have used CRISPR/Cas9 technique to delete the promoter region of MIEN1 in HT-29 CRC cells. RNA-seq experiments revealed that several biological processes related to migration/metastasis were enriched by differentially expressed genes. Genetically modified cells shown decreased migration capacity.
Overall design RNA-seq experiments of WT and genetically modified HT-29 cells.
Contributor(s) Sierra-Diaz DC, Morel A, Ortega-Recalde O, Jiménez KM, Buitrago JC, Valero-Rubio D, Contreras NC, Baquero D, Barco A, Parra-Medina R, Monroy A, Figueroa C, Laissue P
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Submission date Dec 12, 2018
Last update date Dec 14, 2020
Contact name Oscar Javier Ortega-Recalde
Organization name University of Otago
Department Anatomy
Street address 60 Hanover Street
City Dunedin
State/province Otago
ZIP/Postal code 9016
Country New Zealand
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (8)
GSM3510399 HT-29_WT_1
GSM3510400 HT-29_WT_2
GSM3510401 HT-29_WT_3
BioProject PRJNA509662
SRA SRP173340

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE123734_RAW.tar 1.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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