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| Status |
Public on Dec 07, 2018 |
| Title |
Single cell RNA sequencing of Vγ4 and Vγ6 γδT cells from different tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
IL17-producing γδ T cells (γδ T17) mainly develop in the prenatal phase and persist as long-living self-renewing effector cell in all kind of tissues. They express polyclonal T-cell receptors (TCR), comprising public Vγ4+ and Vγ6+ TCRs with germline-like rearrangements. In particular, Vγ6+ T cells have recently been found in a variety of tissues including enthesis, gingiva or skin. However, their exchange between tissues and the mechanisms of tissue-specific adaptation and residency remain poorly understood. Here, we profiled Vγ6+ T cells isolated from thymus, peripheral lymph nodes (pLN) and skin through single-cell RNA-seq technology and compared those to Vγ4+ T cells. Our data demonstrated that Vγ6+ T cells formed highly homogenous cell populations that could be separated by tissue-specific gene expression signatures.
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| Overall design |
Sort Vγ4 and Vγ6 γδT cells from peripheral lymph nodes, ear skin and thymus, then do 3'-RNA single cell sequencing (10x genomics).
|
| Web link |
https://doi.org/10.1016/j.celrep.2019.05.064
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| |
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| Contributor(s) |
Tan L, Ravens S |
| Citation(s) |
31216482 |
| |
| Submission date |
Dec 06, 2018 |
| Last update date |
Jul 30, 2019 |
| Contact name |
Immo Prinz |
| E-mail(s) |
i.prinz@uke.de
|
| Organization name |
University medical center Hamburg Eppendorf
|
| Department |
Institute of Systems Immunology
|
| Street address |
Falkenried 94
|
| City |
Hamburg |
| ZIP/Postal code |
20251 |
| Country |
Germany |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA508628 |
| SRA |
SRP172691 |
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