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| Status |
Public on Jul 15, 2019 |
| Title |
Esrrb function in the extraembryonic ectoderm is required for proper primordial germ cell specification [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
Estrogen related receptor beta (Esrrb) is an orphan nuclear receptor that is required for self-renewal and pluripotency in mouse embryonic stem (ES) cells. However, in the early post-implantation mouse embryo, Esrrb is specifically expressed in the extraembryonic ectoderm (ExE) and plays a crucial role in trophoblast development. In this study, to better understand the function of Esrrb in trophoblast lineage cells, we performed microarray analysis of Esrrb-null mutant versus wild-type mouse embryos. To further characterize the regulatory targets of Esrrb, we treated trophoblast stem (TS) cells with either Esrrb inhibitor DES or vehicle and compared their global gene expression profile via RNA-seq analysis. In addition, to ask whether these target genes are directly regulated by Esrrb, we performed Chromatin immunoprecipitation-sequencing (ChIP-seq) analysis of TS cells using anti-Esrrb antibody.
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| Overall design |
Genome-wide binding of Esrrb in trophoblast stem cells was assessed by ChIP-Seq using anti-Esrrb antibody. An input control was generated concurrently
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| Contributor(s) |
Okamura E, Tam OH, Posfai E, Li L, Cockburn K, Lee CQ, Garner J, Rossant J |
| Citation(s) |
31315026 |
| |
| Submission date |
Dec 05, 2018 |
| Last update date |
Oct 15, 2019 |
| Contact name |
Eiichi Okamura |
| E-mail(s) |
eiokamu@gmail.com
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| Organization name |
Shiga University of Medical Science
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| Department |
Research Center for Animal Life Science
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| Lab |
Department of Stem Cells and Human Disease Models
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| Street address |
Seta, Tsukinowa-cho
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| City |
Otsu |
| State/province |
Shiga |
| ZIP/Postal code |
520-2192 |
| Country |
Japan |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE123363 |
Esrrb function is required for proper primordial germ cell development in presomite stage mouse embryos |
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| Relations |
| BioProject |
PRJNA508492 |
| SRA |
SRP172543 |
