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| Status |
Public on Jul 23, 2019 |
| Title |
Study of the role of IL-12p40-related cytokines in colitis of NEMOIEC-KO mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Disruption of the epithelial barrier is considered a potential cause of inflammatory bowel disease (IBD). In this study, we employed the NEMOIEC-KO mouse model to study the immune mechanisms triggering chronic colitis downstream of an epithelial barrier defect. Colitis in NEMOIEC-KO mice is driven by commensal bacteria sensing through MyD88 signaling. The IL-12p40-related cytokines are induced upon microbial sensing and are known to act critically in promoting intestinal inflammation. Yet, the relative contribution of IL-12 versus IL-23 in eliciting intestinal pathology has been controversial. Using IL-12p40, IL-12p35 and IL-23p19 knockout mice we assessed the functional contribution of IL-12 and IL-23 to intestinal inflammation in the NEMOIEC-KO model.
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| Overall design |
To investigate how p40, p35 or p19 deficiencies affect the inflammatory response in the colon of NEMOIEC-KO mice, we conducted RNA-seq-based transcriptome profiling in the colons of 6-week-old NEMOIEC-KO Il12b-/-, NEMOIEC-KO Il12a-/-, NEMOIEC-KO Il23a-/-, NEMOIEC-KO and Nemofl/fl mice. Five mice per genotype were used as biological replicates.
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| Contributor(s) |
Eftychi C, Wagle P, Pasparakis M |
| Citation(s) |
31350179 |
| |
| Submission date |
Dec 04, 2018 |
| Last update date |
Aug 19, 2019 |
| Contact name |
Manolis Pasparakis |
| E-mail(s) |
pasparakis@uni-koeln.de
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| Organization name |
University of Cologne
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| Department |
Institute for Genetics
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| Lab |
Pasparakis Lab
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| Street address |
Joseph Stelzmann Strasse, 26
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| City |
Cologne |
| State/province |
NRW |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (25)
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| Relations |
| BioProject |
PRJNA508301 |
| SRA |
SRP172453 |
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