|
| Status |
Public on Mar 01, 2019 |
| Title |
Structure and degradation of circular RNAs regulate PKR activation in innate immunity |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
RNase L–mediated rapid degradation of circRNAs is required for PKR activation in innate immune responses and linked to the autoimmune disease systemic lupus erythematosus.
|
| |
|
| Overall design |
Poly(A)+ RNA-seq for gene expression, poly(A)–/Ribo– for circRNA, circSHAPE-MaP and linearSHAPE-MaP for circRNA and linear RNA secondary structure, poly(A)+ RNA-seq for gene expression, poly(A)–/Ribo– for circRNA.
|
| |
|
| Contributor(s) |
Yang L, Chen L, Liu C, Li X, Nan F |
| Citation(s) |
31031002 |
| |
| Submission date |
Nov 26, 2018 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Li Yang |
| E-mail(s) |
liyang_fudan@fudan.edu.cn
|
| Organization name |
Fudan University
|
| Department |
Institutes of Biological Sciences
|
| Street address |
131 Dong-An Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (20)
|
| GSM3489319 |
HeLa ctrl poly(A)-/Ribo- RNA replicate 2 |
| GSM3489320 |
HeLa poly(I:C) poly(A)-/Ribo- RNA replicate 1 |
| GSM3489321 |
HeLa poly(I:C) poly(A)-/Ribo- RNA replicate 2 |
| GSM3489322 |
HeLa RNaseL KO ctrl poly(A)+ RNA |
| GSM3489323 |
HeLa RNaseL KO poly(I:C) poly(A)+ RNA |
| GSM3489324 |
HeLa RNaseL KO ctrl poly(A)-/Ribo- RNA replicate 1 |
| GSM3489325 |
HeLa RNaseL KO ctrl poly(A)-/Ribo- RNA replicate 2 |
| GSM3489326 |
HeLa RNaseL KO poly(I:C) poly(A)-/Ribo- RNA replicate 1 |
| GSM3489327 |
HeLa RNaseL KO poly(I:C) poly(A)-/Ribo- RNA replicate 2 |
| GSM3489328 |
HeLa wt ctrl poly(A)+ RNA replicate 1 |
| GSM3489329 |
HeLa wt ctrl poly(A)+ RNA replicate 2 |
| GSM3489330 |
HeLa wt EMCV poly(A)+ RNA replicate 1 |
| GSM3489331 |
HeLa wt EMCV poly(A)+ RNA replicate 2 |
| GSM3489332 |
HeLa wt ctrl poly(A)-/Ribo- RNA replicate 1 |
| GSM3489333 |
HeLa wt ctrl poly(A)-/Ribo- RNA replicate 2 |
| GSM3489334 |
HeLa wt EMCV poly(A)-/Ribo- RNA replicate 1 |
| GSM3489335 |
HeLa wt EMCV poly(A)-/Ribo- RNA replicate 2 |
|
| Relations |
| BioProject |
PRJNA507020 |
| SRA |
SRP170763 |
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