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| Status |
Public on Feb 13, 2019 |
| Title |
Development of gene expression signatures for progression and regression of atherosclerosis |
| Organism |
Oryctolagus cuniculus |
| Experiment type |
Expression profiling by array
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| Summary |
To further study the important genes related with the diagnosis and prognosis of atherosclerosis, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the atherosclerosis. we established a rabbit model of progression and regression of atherosclerosis through balloon injury and high-cholesterol diet and normal diet. Rabbit abdominal aortas from atherosclerosis among control, model group (progression) and recovery groups (regression) were collected. We found that the differentially expressed genes mainly enriched in four clusters and genes associated with inflammation and extracellular matrix were returned to or close to normal levels much earlier than the genes associated with metabolism and sarcoplasmic proliferation, they kept down-regulation or up-regulation for a long time after normal diet. Expression of four genes (LPL，BMP7，MCP2 and SST) from this signature was quantified in the same RNA samples by real-time PCR and maybe used as novel biomarkers and targets for improving the diagnosis and prognosis of atheroscleross in the future.
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| Overall design |
we established a rabbit model of progression and regression of atherosclerosis through balloon injury and high-cholesterol diet and normal diet. Rabbit abdominal aortas from atherosclerosis among control, model group (progression) and recovery groups (regression) were collected for gene chip.
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| Contributor(s) |
Yu X, Yu Y |
| Citation(s) |
30958112 |
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| Submission date |
Nov 19, 2018 |
| Last update date |
May 15, 2019 |
| Contact name |
Xiaolan Yu |
| E-mail(s) |
xiaolanyu89@163.com
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| Phone |
+86-21-34204902
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| Organization name |
Shanghai JiaoTong Univerisity
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| Street address |
800 Dongchuan RD, Minhang District
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| City |
Shanghai |
| ZIP/Postal code |
201100 |
| Country |
China |
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| Platforms (1) |
| GPL13288 |
Agilent-020908 Oryctolagus cuniculus (Rabbit) Oligo Microarray (Probe Name version) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA505986 |
