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Series GSE122635 Query DataSets for GSE122635
Status Public on Dec 31, 2018
Title LRP5 and LRP6 are required for maintaining self-renewal and differentiation of hematopoietic stem cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The canonical Wnt signaling pathway has been demonstrated as a critical role in the self-renewal, proliferation and differentiation of Hematopoietic Stem Cells (HSCs), but the functions are indeterminacy in adult HSCs since the different experimental systems using gain- or loss- functions mice models. Low-density lipoprotein receptor-related proteins 5 and 6 (LRP5 and LRP6) are important co-receptors in the canonical Wnt/β-catenin pathway. In this study, the knockout mice models were established for adult HSC investagation via conditional ablation of LRP5 and LRP6 in adult hematopoiesis by Vav-Cre Loxp system. We found the HSC numbers were diminished due to the decreased proliferation and cell cycle. To investigate the molecular mechanisms, RNA-seq was performed using HSC cells isolated from WT and dKO mice.
Overall design HSC (CD34-Flt3- Lin-ScaI+C-Kithigh cells) purified by FACS sorting
Contributor(s) Yu S, Liu J
Citation(s) 30668923
Submission date Nov 16, 2018
Last update date Apr 01, 2019
Contact name Shuyang Yu
Organization name China Agricultural University
Department College of Biological Sciences
Street address 2 Yuanmingyuan West Road
City Beijing
ZIP/Postal code 100193
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (5)
GSM3476854 WT rep1
GSM3476855 WT rep2
GSM3476856 Lrp5/6 dKO rep1
BioProject PRJNA505821
SRA SRP169107

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122635_RAW.tar 2.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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