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| Status |
Public on Nov 16, 2018 |
| Title |
Effects of NSUN2 deficiency on the mRNA 5-methylcytosine modification and gene expression profile in HEK293 cells (RNA-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Aim: To study the biological function of NSUN2 in regulating gene expression and cell proliferation.
Materials & methods: The NSUN2 gene was knocked down in HEK293 cells via CRISPR/Cas9 system. Gene expression were assessed using RNA-Seq.
Results: A total of 790 differentially expressed genes (DEGs) were identified.
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| |
|
| Overall design |
mRNA profiles of wild type (WT) and NSUN2-/- HEK293 cells were generated by deep sequencing, in triplicate.
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| |
|
| Contributor(s) |
Cui H, Sun Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 12, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Zhen Sun |
| Organization name |
Yangzhou University
|
| Street address |
Wenhui
|
| City |
Yangzhou |
| ZIP/Postal code |
225009 |
| Country |
China |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA505172 |
| SRA |
SRP168405 |
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