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Series GSE122409 Query DataSets for GSE122409
Status Public on Apr 22, 2020
Title High CD26 and low CD94 expression identifies an IL-23 responsive V(delta)2+ T cell subset with a MAIT cell-like transcriptional profile
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary In this study, we find that CD26 and CD94 co-expression identifies transcriptionally distinct V(delta)2+ T cell subsets that vary in cytokine-responsiveness and cytotoxic potential, with a direct relationship between the loss of CD26 surface expression and upregulation of GzmB. These subsets differ in the expression of key transcriptional regulators including Tbet, Eomes and Hobit, and exist independently of classical memory markers. Analysis of cord blood samples confirms that CD26+ V(delta)2+ T cells predominate at birth, and we find evidence that IL-23 exposure and CD26 ligation may contribute to the generation of CD26- cytotoxic V(delta)2+ T cells in adults.
Overall design Samples 1-12: Freshly isolated PBMC from 3 healthy donors were stained for CD3, V(delta)2 TCR, CD26 and CD94. Cells were identified as Live/Dead negative, CD3+ V(delta)2+ cells. Four populations (CD26+CD94lo, CD26+CD94hi, CD26-CD94hi, CD26-CD94lo) were sorted from these PBMCs. Transcriptional profiling and differential gene expression was performed using RNA sequencing. Samples 13-28: For the V(delta)2 expansion RNA sequencing, individual CD26/CD94 V(delta)2 subsets were sorted from a single blood draw and expanded in parallel with (gamma-delta)-depleted PBMC, zoledronate, IL-2 and (if applicable) IL-23. At day 14 post-stimulation, cells were surface stained and live, CD3+ V(delta)2+ cells were sorted. Transcriptional profiling and differential gene expression was performed using RNA sequencing.
Contributor(s) Wragg KM, Tan H, Kristensen AB, Nguyen-Robertson CV, Kelleher AD, Parsons MS, Wheatley AK, Berzins SP, Pellicci DG, Kent SJ, Juno JA
Citation(s) 32553157
Submission date Nov 10, 2018
Last update date Jul 22, 2020
Contact name Hyon Xhi Tan
Organization name The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity
Department Department of Microbiology and Immunology
Street address 792 Elizabeth St
City Melbourne
State/province Victoria
ZIP/Postal code 3010
Country Australia
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (28)
GSM3466250 Donor 1 CD26+ CD94hi (D1 DP)
GSM3466251 Donor 1 CD26- CD94hi (D1 94)
GSM3466252 Donor 1 CD26+ CD94lo (D1 26)
BioProject PRJNA505043
SRA SRP168396

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Supplementary file Size Download File type/resource
GSE122409_Degust_Voom_Limma.csv.gz 1.4 Mb (ftp)(http) CSV
GSE122409_Degust_Voom_Limma_-_IL23_expansion.csv.gz 2.4 Mb (ftp)(http) CSV
GSE122409_Raw_counts_-_IL23_expansion.txt.gz 532.5 Kb (ftp)(http) TXT
GSE122409_Raw_counts_all_subsets.txt.gz 326.5 Kb (ftp)(http) TXT
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