Expression profiling by high throughput sequencing
Summary
The goal of this project is to generate transcriptome profiling of Lgr5-GFP high intestinal stem cells (High)/Lgr5-GFP low progenitors (Low) and CD24+C-kit+ Paneth (PA) cells for a systemic analysis of cellular pathways involved in responses to fasting and aging.
Overall design
Intestinal crypt cells were isolated from mice fed ad libitum or fasted for 24 hours, at young and old ages. The goal is to compare the differential responses of three cell types to fed/fasiting states under the effects of aging. Please note that the original RNA-Seq libraries were sequenced three times, the first run was submitted as mm9-mapped bam files and the other two runs are provided as fastq files. All analytical steps were performed against mm10.
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