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| Status |
Public on Jul 10, 2011 |
| Title |
Identification of Plasmodium vivax genes whose expression is spleen-dependent |
| Organism |
Plasmodium vivax |
| Experiment type |
Expression profiling by array
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| Summary |
The goal of this study is to identify P. vivax genes whose expression is dependent on the intact spleen in experimental infections in Aotus monkeys.
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| Overall design |
These studies were carried-out at the facilities of the “Fundación Centro de Primates de la Universidad del Valle”, Cali, Colombia and in the “Barcelona Centre for International Health Resarch” - CRESIB, Barcelona, Spain. This protocol was approved from the Ethical Committees of both Centres. A total of 4 Aotus lemurinus griseimembra naive animals were used in these experiments. Three animals were splenectomized whereas another had an intact spleen. A donor monkey was infected with P. vivax Sal-I strain and after peak parasitemias appeared a time-series infections into Sp-1, Sp-2, Sp-3, and Sp+2 animals were performed. Parasites from each infection were obtained from peripheral blood, monkey leukocytes were removed by MidMacs and only purified schizont stages were used for RNA extractions. Dual-hybridizations Cy3/Cy5 comparing the global expression of parasites obtained from different infections (Cy5) with a reference pool PvSp-1 obtained from splenectomized monkeys from CDC (PvSp-1) were perfomed using an Agilent's 60-mer platform representing the complete coding genome of P. vivax (1 oligonucleotide/2 kb coding sequences) GPL6667
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| Contributor(s) |
Fernandez-Becerra C, Vencio RN, Herrera S, Arevalo-Herrera M, Barnwell J, del Portillo HA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 20, 2008 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Hernando A del Portillo |
| E-mail(s) |
hernandoa.delportillo@cresib.cat
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| Organization name |
Barcelona Centre for International Health Research
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| Lab |
malaria
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| Street address |
rosello, 132 (4a)
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| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
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| Platforms (1) |
| GPL6667 |
P. vivax exon array v1.0 using oligos designed by Z. Bozdech and G. Hu |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA113467 |
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