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Series GSE121593 Query DataSets for GSE121593
Status Public on Nov 30, 2018
Title The role of oxygen in regulating microRNAs that control the placental renin-angiotensin system
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The human placental renin-angiotensin system (RAS) genes encoding for prorenin (REN), angiotensinogen (AGT), prorenin receptor (ATP6AP2) and angiotensin II type I (AGTR1) receptor are upregulated in early gestation and affect placental development. At this time, the placental oxygen tension is at its lowest (1-3%). Some miRNAs predicted to target the RAS are downregulated in early gestation. Our hypothesis is that the low oxygen tension in early pregnancy suppresses expression of miRNAs that target the placental RAS; thus expression of these RAS genes are upregulated.
Ten miRNAs predicted to target RAS mRNAs were downregulated in response to 1% vs. 20% oxygen, and six miRNAs were decreased in cells cultured in 1% vs. 5% oxygen.
Overall design HTR-8/SVneo cells, a human extravillous trophoblast cell line, were cultured in 1%, 5% and 20% oxygen tensions (to reflect the oxygen tensions of the chorionic plate, decidua and standard culture conditions respectively) for 48 h (n=3 in triplicate). Any differences in expression of miRNAs between these groups were determined. Affymetrix miRNA microarray analysis was conducted, which detected up to 2006 miRNAs. Statistically different levels of expression were determined. MiRNAs predicted to target the placental RAS were identified using a variety of databases (,,, and Differences in miRNA expression measured on the microarray were confirmed for some miRNAs by RT-PCR (qPCR, n=3 in triplicate for all groups).
Contributor(s) Arthurs AL, Lumbers ER, Delforce SJ, Mathe A, Morris BJ, Pringle KG
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Submission date Oct 22, 2018
Last update date Dec 02, 2018
Contact name Kirsty G Pringle
Phone 40420329
Organization name University of Newcastle
Department HMRI
Lab Level 3 East
Street address 1 Kookaburra Circuit
City Newcastle
State/province NSW
ZIP/Postal code 2305
Country Australia
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (9)
GSM3439850 Extravillous trophoblast 1% O2 #1
GSM3439851 Extravillous trophoblast 5% O2 #1
GSM3439852 Extravillous trophoblast 20% O2 #1
BioProject PRJNA497848

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Supplementary file Size Download File type/resource
GSE121593_RAW.tar 6.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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