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| Status |
Public on Jan 02, 2019 |
| Title |
RNA-seq in scraped colon epithelium after deletion of Synbindin in mice in colitis-associated cancer model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We previously identified Synbindin promoted gastric and colon cancer development by activating ERK on the Golgi in vitro. Advanced in vivo evidence is required.In this study, we genetically manipulated Synbindin expression in the intestinal epithelium using loss-of-function mouse models and subject them to AOM/DSS induced colitis-associated cancer model. At the end of the model, mice were sacrificed and colon tumors and adjacent colon epithelium were scraped and used for RNA-seq.
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| Overall design |
Colon mRNA profiles of Synbindinflox and SynbindinΔIEC mice were generated by deep sequencing, in triplicate,using illumina Hiseq.
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| Contributor(s) |
Ai L |
| Citation(s) |
30561033 |
| |
| Submission date |
Oct 22, 2018 |
| Last update date |
Mar 01, 2019 |
| Contact name |
Luoyan Ai |
| E-mail(s) |
storysparrow@126.com
|
| Phone |
15821953541
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| Organization name |
Shanghai Jiao Tong University
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| Department |
Division of Gastroenterology and Hepatology
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| Lab |
Shanghai Institute of Digestive Disease
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| Street address |
145 Middle Shandong Road
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| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200001 |
| Country |
China |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA497795 |
| SRA |
SRP166276 |
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