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| Status |
Public on Aug 11, 2019 |
| Title |
Virus- and interferon alpha-induced transcriptomes of cells from the microbat Myotis daubentonii |
| Organism |
Myotis daubentonii |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
These RNA-Seq data set was generated to study the virus- and interferon alpha-induced immune response of M. daubentonii (microbat) kidney cells. For the virus challenge, a RVFV clone (Clone-13) was used.
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| Overall design |
Total RNA from control (mock), interferon-alpha (IFN) or Rift Valley fever virus Clone 13 (Clone13) infected cells were isolated at two time points (6h, 24h) post treatment/infection. The quality and integrity of the RNA was controled by a Agilent 2100 Bioanalyzer followed by deep sequencing. For each total RNA sample cDNA libraries were prepared utilizing the Illumina Ribo-Zero rRNA Removal Kit for human/mouse/rat (hereafter rRNA-). Importantly, no polyA selection was applied. Overall, 18 rRNA- libraries were sequenced on six HiSeq 2500 lanes with 51 cycles resulting in 60-70 million strand-specific single-end reads per sample. The complete experiment was performed in three independent, biological replicates. As no reference genome for M. daubentonii was available when conducting this study, we used the genome of the closely related microbat species M. lucifugus as a reference for mapping and quantification.
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| Contributor(s) |
Hölzer M, Schoen A, Wulle J, Müller M, Drosten C, Marz M, Weber F |
| Citation(s) |
31465999, 32289119 |
| |
| Submission date |
Oct 16, 2018 |
| Last update date |
Apr 20, 2020 |
| Contact name |
Martin Hölzer |
| E-mail(s) |
martin.hoelzer@uni-jena.de
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| Phone |
+4916092675865
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| Organization name |
University of Jena
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| Department |
Faculty of Mathematics and Informatics
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| Lab |
RNA Bioinformatics and High-Throughput Analysis
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| Street address |
Leutragraben 1
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| City |
Jena |
| State/province |
Thuringia |
| ZIP/Postal code |
07743 |
| Country |
Germany |
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| Platforms (1) |
| GPL25678 |
Illumina HiSeq 2500 (Myotis daubentonii) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA496612 |
| SRA |
SRP165844 |
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