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Series GSE121251 Query DataSets for GSE121251
Status Public on Apr 10, 2019
Title Foxp1 is critical for the maintenance of regulatory T-cell homeostasis and suppressive function
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Regulatory T (Treg) cells play central roles in maintaining immune homeostasis and self-tolerance. However, the molecular mechanisms underlying Treg cell homeostasis and suppressive function are still not fully understood. Here, we report that the deletion of another P subfamily members of the forkhead box (Foxp) subfamily member Foxp1 in Treg cells led to increased numbers of activated Treg (aTreg) cells at the expense of quiescent Treg cells, and also resulted in impaired Treg suppressive function. Mice with Foxp1-deficient Treg cells developed spontaneous inflammatory disease with age; they also had more severe inflammatory disease in colitis and experimental autoimmune encephalomyelitis (EAE) models. Mechanistically, we found that Foxp1 bound to the conserved noncoding sequence 2 (CNS2) element of the Foxp3 locus and helped maintain Treg suppressive function by stabilizing the Foxp3 expression. Furthermore, we found that Foxp1 and Foxp3 coordinated the regulation of cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) expression levels. Taken together, our study demonstrates that Foxp1 plays critical roles in both maintaining Treg cell quiescence during homeostasis and regulating Treg suppressive function.
Overall design Spleen and peripheral lymph nodes were harvested in Foxp3-YFP-Cre and Foxp1fl/fl Foxp3-YFP-Cre mice. For RNAseq, We have four samples, WT rTreg, WT aTreg, cKO rTreg, cKO aTreg and 2 replicates for each sample.
Contributor(s) Wang H, Ren J, Han L, Tang J
Citation(s) 31125332
Submission date Oct 15, 2018
Last update date Aug 28, 2019
Contact name Jinyi Tang
Phone 17721241521
Organization name IPS
Lab Wang Haikun
Street address Yueyang road 320
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (8)
GSM3428895 WT rTreg 1
GSM3428896 WT rTreg 2
GSM3428897 WT aTreg 1
BioProject PRJNA496482
SRA SRP165748

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Supplementary file Size Download File type/resource
GSE121251_Counts.mRNA.foxp1.txt.gz 456.0 Kb (ftp)(http) TXT
GSE121251_FPKM.mRNA.foxp1.txt.gz 934.8 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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