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Series GSE121250 Query DataSets for GSE121250
Status Public on May 01, 2019
Title ZIKV infection induces robust Th1-like Tfh cell and long-term protective antibody responses in immunocompetent mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Induction of long-lived antibody responses during infection or vaccination is often essential for subsequent protection, but the relative contributions of T follicular helper (Tfh) cells and T helper 1 (Th1) cells for induction of antigen specific antibody responses to viruses are unclear. Here, we establish an acute Zika virus (ZIKV) infection model in immunocompetent mice, and show that ZIKV infection elicits robust Th1-like Tfh cell and protective antibody responses. While these Th1-like Tfh cells share phenotypic and transcriptomic profiles with both Tfh and Th1 cells, they also have unique surface markers and gene expression characteristics, and are dependent on T-bet for their development. Th1-like Tfh cells, but not Th1 cells, are essential for class switching of ZIKV-specific IgG2c antibodies and maintenance of long-term neutralizing antibody responses. Our study suggests that specific modulation of the Th1-like Tfh cell response during infection or vaccination may augment the induction of antiviral antibody response to ZIKV and other viruses.
Overall design IFN-γ-YFP reporter mice were infected by ZIKV with anti-IFNα/β receptor blocking one day before. Spleens and dLNs were harvested 7 dpi to sort. For RNAseq, We have three samples, Tfh(IFN-γ- Tfh), Th1-like Tfh(IFN-γ+ Tfh), Th1 and 2 replicates for each sample.
Web link
Contributor(s) Wang H, Tang J
Citation(s) 31455769
Submission date Oct 15, 2018
Last update date Sep 28, 2019
Contact name Jinyi Tang
Phone 17721241521
Organization name IPS
Lab Wang Haikun
Street address Yueyang road 320
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM3428889 RNA_Tfh_rep1
GSM3428890 RNA_Tfh_rep2
GSM3428891 RNA_Th1-like-Tfh_rep1
BioProject PRJNA496481
SRA SRP165746

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Supplementary file Size Download File type/resource
GSE121250_Counts.mRNA.Tfh.txt.gz 547.5 Kb (ftp)(http) TXT
GSE121250_FPKM.mRNA.Tfh.txt.gz 724.2 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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