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| Status |
Public on Jan 15, 2019 |
| Title |
Oligodendrocyte progenitor cells become regionally diverse and heterogeneous with age |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Oligodendrocyte progenitor cells (OPCs), which differentiate into myelinating oligodendrocytes during central nervous system (CNS) development, are the main proliferative cells in the adult brain. OPCs are conventionally considered a homogeneous population, particularly with respect to their electrophysiological properties, but this has been debated. We show, by using single-cell electrophysiological recordings, that OPCs start out as a homogeneous population, but become functionally heterogeneous, varying both within and between brain regions and with age. These electrophysiological changes in OPCs correlate with the differentiation potential of OPCs; thus, they may underlie the differentiational differences in OPCs between regions and likewise differentiation failure with age.
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| Overall design |
Total RNA profiles of oligodendrocyte precursor cells isolated from 3 to 4 wild-type mice at different time points, using SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian.
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| Contributor(s) |
Dietmann S, Evans KA, Kamen Y, Káradóttir RT |
| Citation(s) |
30654924 |
| |
| Submission date |
Oct 10, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Sabine Dietmann |
| Organization name |
Washington University School of Medicine
|
| Street address |
4565 McKinley Avenue
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA495612 |
| SRA |
SRP164927 |
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