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| Status |
Public on Oct 10, 2018 |
| Title |
Expression data from rat bone marrow mesenchymal stem cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Mesenchymal stem cells (MSCs) are involved in tissue homeostasis and repair, and can be impaired in subjects with high fat diet (HFD)-induced metabolic disorders.
We used microarrays to detail the global programme of gene expression bone marrow MSCs (BMSCs) underlying HFD-induced progression of metabolic disorders in rat and identified distinct classes of differentiatilly expressed genes during this process.
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| Overall design |
Six-week old Sprague-Dawley (SD) rats were randomized to normal diet (ND) and high-fat diet (HFD) groups, fed a normal diet (ND, 100% basal diet) and a customized high-fat diet (60% basal diet, 10% sucrose, 20% lard and 10% egg yolk powder), respectively. The total mRNA of BMSCs (P1) from both groups (n= 3 per group) at the specified feeding time points (1, 2 and 4 months) was purified and hybridized on Affymetrix microarrays. BMSCs were isolated and characterized for the expression of cell surface markers before RNA extraction, to improve the accuracy of expression profiles.
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| Contributor(s) |
Bi J, Li Q |
| Citation missing |
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| Submission date |
Oct 09, 2018 |
| Last update date |
Oct 11, 2018 |
| Contact name |
XIN FU |
| E-mail(s) |
fuxin@psh.pumc.edu.cn
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| Phone |
86-010-88772040
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| Organization name |
Plastic Surgery Hospital (Institute), Peking Union Medical College & Chinese Academy of Medical Sciences
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| Department |
Research Center
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| Street address |
33 Ba-Da-Chu Road
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| City |
Beijing |
| ZIP/Postal code |
100144 |
| Country |
China |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA495385 |
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