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| Status |
Public on Jul 28, 2019 |
| Title |
Amino acids availability and subsequent metabolic reprogramming sustain endocrine resistance in breast cancer (mRNA) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The majority of breast tumours express oestrogen receptor (ER) and receive endocrine therapy (ET) as standard care. Despite its efficacy, ~40% of women relapse with endocrine therapy-resistant (ETR) disease. A global transcription analysis and subsequent integrative approaches of ETR cells showed a deregulated node between miR-23b-3p and the amino acids transporter SLC6A14. Mechanistic analysis supports a model in which such deregulation impairs amino acids metabolism in the ETR cells with subsequent activation of autophagy and enhanced aspartate and glutamate import mediated by the SLC1A2 transporter. The clinical significance of these findings was validated by multiple orthogonal approaches in a large cohort of ET-treated patients, in patient-derived xenografts and in in vivo experiments. Targeting such reprogramming impairs the aggressive features of ETR cells and offers predictive biomarkers and potential targetable pathways to be exploited to combat or delay ETR in ER positive breast cancers.
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| Overall design |
This dataset consist in 9 samples obtained from 3 independent RNA extactions of parental cell line MCF7 (model of breast adenocarcinoma), in presence (MCF7+E2) or absence (MCF7-E2) of oestrogen (E2), and their derivative LTED (Long Term Estrogen Deprived)
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| Contributor(s) |
Ferracin M, Morandi A, Ramazzotti M |
| Citation(s) |
31269432 |
| |
| Submission date |
Oct 08, 2018 |
| Last update date |
Jul 30, 2019 |
| Contact name |
Matteo Ramazzotti |
| E-mail(s) |
matteo.ramazzotti@unifi.it
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| Organization name |
University of Florence
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| Department |
Experimental and Clinical Biomedical Sciences
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| Street address |
viale Morgagni 50
|
| City |
Firenze |
| ZIP/Postal code |
50134 |
| Country |
Italy |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (9)
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| GSM3421728 |
Breast adenocarcinoma cell line MCF7 – Long Term Estrogen Deprived (LTED) – rep1 |
| GSM3421729 |
Breast adenocarcinoma cell line MCF7 – Long Term Estrogen Deprived (LTED) – rep2 |
| GSM3421730 |
Breast adenocarcinoma cell line MCF7 – Long Term Estrogen Deprived (LTED) – rep3 |
| GSM3421731 |
Breast adenocarcinoma cell line MCF7 – cultured with oestrogen – rep1 |
| GSM3421732 |
Breast adenocarcinoma cell line MCF7 – cultured with oestrogen – rep2 |
| GSM3421733 |
Breast adenocarcinoma cell line MCF7 – cultured with oestrogen – rep3 |
| GSM3421734 |
Breast adenocarcinoma cell line MCFT – cultured without oestrogen – rep1 |
| GSM3421735 |
Breast adenocarcinoma cell line MCFT – cultured without oestrogen – rep2 |
| GSM3421736 |
Breast adenocarcinoma cell line MCFT – cultured without oestrogen – rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE120931 |
Amino acids availability and subsequent metabolic reprogramming sustain endocrine resistance in breast cancer |
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| Relations |
| BioProject |
PRJNA495051 |
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