|
| Status |
Public on Oct 18, 2018 |
| Title |
Characterisation by Next generation Capture-C of Pax6 locus in three mouse cell lines (MV+, RAG and β-TC3 cells). |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
NG-Capture-C profiling of chromatin interactions (using DpnII 3C and Oligo nucleotide sequence capture followed by Next generation sequencing) of enhancers, promoters and CTCF/Rad21 binding sites throughout a ~600kb region of the mouse Pax6 locus in three expression states, High (β-TC3), On (MV+) and OFF (RAG). β-TC3 cell are a mouse pancreatic β cell derived from mouse insulinoma, MV+ a lens epithelium cell line and RAG a mouse kidney adenocarcinoma cell line.
|
| |
|
| Overall design |
Three cell lines where analysed and two biological replicates generated for each, with two separte panels of capture oligos.
|
| |
|
| Contributor(s) |
Buckle A, Brackley C, Boyle S, Marenduzzo D, Gilbert N |
| Citation(s) |
30344096, 37537334 |
| |
| Submission date |
Sep 30, 2018 |
| Last update date |
Sep 20, 2023 |
| Contact name |
nick gilbert |
| E-mail(s) |
nick.gilbert@ed.ac.uk
|
| Phone |
00 44 131 651 8551
|
| Organization name |
University of Edinburgh
|
| Department |
MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine
|
| Street address |
Crewe Rd
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
|
| Platforms (2) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (20)
|
|
| Relations |
| BioProject |
PRJNA494025 |
| SRA |
SRP162962 |
Less...
More...