|
Status |
Public on Dec 27, 2018 |
Title |
The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
Human primordial germ cells (hPGCs) are the first embryonic progenitors in the germ cell lineage, yet the molecular mechanisms required for hPGC formation are not well characterized. To identify regulatory regions in hPGC development, we used the assay for transposase-accessible chromatin using sequencing (ATAC-seq) to systematically characterize regions of open chromatin in hPGCs and hPGC-like cells (hPGCLCs) differentiated from human embryonic stem cells (hESCs). We discovered regions of open chromatin unique to hPGCs and hPGCLCs that significantly overlap with TFAP2C-bound enhancers identified in the naive ground state of pluripotency. Using CRISPR/Cas9, we show that deleting the TFAP2C-bound naive enhancer at the OCT4 locus (also called POU5F1) results in impaired OCT4 expression and a negative effect on hPGCLC identity.
|
|
|
Overall design |
26 ATAC-seq libraries: 9 ATAC-seq libraries from primed hESCs, 4 ATAC-seq libraries from iMeLC induced from primed hESCs, 3 ATAC-seq libraries from hPGCLCs induced from primed hESCs, 6 ATAC-seq libraries from sorted hPGCs, 4 ATAC-seq libraries from liver, lung, skin and heart cells from human embryo.
|
|
|
Contributor(s) |
Chen D, Liu W |
Citation(s) |
30590035 |
|
Submission date |
Sep 28, 2018 |
Last update date |
Mar 27, 2019 |
Contact name |
Wanlu Liu |
Organization name |
Zhejiang University
|
Department |
Zhejiang University - University of Edinburgh Institute
|
Lab |
Wanlu Liu
|
Street address |
718 East Haizhou Rd.,
|
City |
Haining |
State/province |
Zhejiang |
ZIP/Postal code |
314400 |
Country |
China |
|
|
Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
Samples (26)
|
|
Relations |
BioProject |
PRJNA493830 |
SRA |
SRP162878 |