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| Status |
Public on Apr 23, 2019 |
| Title |
Gene expression profiling of neural crest progenitor cultures derived from human embryonic stem cells carrying nonsense mutations in the Polycomb gene ASXL1 [HOM] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We observed impaired neural crest cell differentiation from human embryonic stem cells that harbor nonsense mutations in the Polycomb gene ASXL1. To investigate the underlying molecular mechanisms, we harvested neural crest differentiation cultures from wildtype and homozygous ASXL1 mutant lines at day 7 of a neural crest differentiation protocol and performed total RNA sequencing using the Illumina NextSeq system. Analyses of global transcriptomes revealed profound changes between wildtype and mutant cultures, and we identified downregulation of several genes of the neural crest regulatory network, most prominently of the transcription factor ZIC1.
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| Overall design |
Global mRNA profiles of day 7 neural crest differentiation cultures derived from different human embryonic stem cell lines (three replicates of wildtype controls, and 7 replicates from 3 different clones harbouring 500 bp deletions/inversions in the central part of ASXL1) were generated on the Illumina NextSeq system.
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| Contributor(s) |
Matheus F, Simon L, Drukker M |
| Citation(s) |
31006630 |
| |
| Submission date |
Sep 19, 2018 |
| Last update date |
Jun 18, 2019 |
| Contact name |
Friederike Matheus |
| Organization name |
Helmholtz Center Munich
|
| Department |
Institute for Stem Cell Research
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| Street address |
Ingolstädter Landstrasse 1
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| City |
München |
| ZIP/Postal code |
85764 |
| Country |
Germany |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE127269 |
Gene expression profiling of neural crest progenitor cultures derived from human embryonic stem cells carrying nonsense mutations in the Polycomb gene ASXL1 |
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| Relations |
| BioProject |
PRJNA491940 |
| SRA |
SRP162158 |
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