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| Status |
Public on Sep 01, 2019 |
| Title |
Macrophage Ad5f35 PhenoMap |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Human monocyte derived macrophages (GM-CSF differentiated) were stimulated with control media, IFNy/LPS (M1), IL4 (M2), empty vector Ad5f35 (MOI 1000), or anti-HER2 CAR Ad5f35 (MOI 1000). RNA was harvested 48 hours post transduction with Ambion RiboPure RNA Purification Kit (Thermo Fisher) and RNA-Seq libraries were generated using TruSeq RNA Library Prep Kit (Illumina). Libraries were sequenced on 75bp single-end reads using a NextSeq (Illumina).
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| Overall design |
Examination of the transcriptome of control (n=4), M1 (n=3), M2 (n=4), empty vector adenovirally transduced (n=4), or anti-HER2 CAR adenovirally transduced human macrophages (n=4). Each n represents a unique normal human donor.
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| |
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| Contributor(s) |
Klichinsky M, Lu XM, Gill S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 17, 2018 |
| Last update date |
Sep 01, 2019 |
| Contact name |
Paul Zhiping Wang |
| E-mail(s) |
upenn.bic@gmail.com
|
| Phone |
2155733117
|
| Organization name |
Bioinformatics Core, IBI, UPen
|
| Street address |
3440 Market St
|
| City |
Philadelphia |
| State/province |
pa |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA491520 |
| SRA |
SRP161956 |
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