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| Status |
Public on Jul 22, 2019 |
| Title |
CDK12 catalytic activity is rate-limiting for RNAPII processivity on core DNA replication genes and G1/S progression (3' RNA) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study we characterized the importance of CDK12-kinase activity in cell cycle regulation, using CDK12 anolog-sentive cells. Inhibition of analog-sensitive CDK12 reveals its catalytic activity is necessary for optimal G1/S progression. Mechanistically, CDK12 regulates transcription of core DNA replication genes and affects timely assembly of pre-replication DNA complex on chromatin. We have performed 3’-end RNA-sequencing after CDK12 inhibition and identified that the expression of core DNA replication genes were affected. To investigate further, we carried out nuclear RNA-seq coupled with ChIP-seq, and demonstrated that CDK12 regulates RNAPII processivity of core DNA replication genes and optimal G1/S progression.
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| Overall design |
3'end RNA seq was performed in three biological replicates, after treatment with or without 5 µM 3-MB-PP1 for 5 h in serum synchronised AS CDK12 HCT116 cells. Total number of samples analyzed were six.
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| Contributor(s) |
Manavalan AC, Pilarova K, Kluge M, Bartholomeeusen K, Oppelt J, Paruch K, Krejci L, Friedel CC, Blazek D |
| Citation(s) |
31347271, 37456509 |
| |
| Submission date |
Sep 17, 2018 |
| Last update date |
Sep 12, 2023 |
| Contact name |
Caroline Friedel |
| Organization name |
Ludwig-Maximilians-Universität München
|
| Department |
Institut für Informatik
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| Street address |
Amalienstr. 17
|
| City |
München |
| ZIP/Postal code |
80333 |
| Country |
Germany |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE120072 |
CDK12 catalytic activity is rate-limiting for RNAPII processivity on core DNA replication genes and G1/S progression |
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| Relations |
| BioProject |
PRJNA491605 |
| SRA |
SRP162070 |
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