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| Status |
Public on Sep 13, 2020 |
| Title |
Defining Essential Enhancer for Pluripotent stem cells using Features Oriented CRISPR-Cas9 Screen (RNA-Seq 1) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Cis Regulatory Elements (CREs) regulate the expression of the genes in their genomic neighborhoods and influence cellular processes such as cell-fate maintenance and differentiation. To date, there remain major gaps in the functional characterization of CREs and the identification of its target genes in the cellular native environment. In this study, we performed a Features Oriented CRISPR Utilized Systematic (FOCUS) screen of Oct4-bound CREs using CRISPR/Cas9 to identify functional CREs important for pluripotency maintenance in mouse ES cells.
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| Overall design |
RNA profiling of shLrrc31 mESCs(2 shRNAs) and shEV mESCs(2 EVs), RNA-seq was performed in biology replicates
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| Contributor(s) |
Wang HF, Warrier T, El Farran CA, Li H, Loh YH |
| Citation(s) |
33113365 |
| |
| Submission date |
Sep 13, 2018 |
| Last update date |
Dec 13, 2020 |
| Contact name |
Chadi El Farran |
| Organization name |
Institute of Molecular and Cell Biology
|
| Lab |
Epigenetics and Cell Fates Laboratory
|
| Street address |
61 Biopolis Drive
|
| City |
Singapore |
| ZIP/Postal code |
138673 |
| Country |
Singapore |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE119951 |
Defining Essential Enhancer for Pluripotent stem cells using Features Oriented CRISPR-Cas9 Screen |
|
| Relations |
| BioProject |
PRJNA490762 |
| SRA |
SRP161719 |
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