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| Status |
Public on Sep 13, 2018 |
| Title |
Transcriptome differences between primary brown adipocytes of wildtype and UCP1-KO mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We analyzed coding transcript abundance in fully differentiated, primary brown adipocytes from murine, interscapular brown adipose tissue of wildtype and UCP1-KO mice.
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| Overall design |
We prepared primary cells from the stromal-vascular fraction of murine interscapular brown fat and differentiated these in vitro into brown adipocytes. Three independent cultures each were generated from wildtype 129SvEvS1 mice and UCP1-KO mice on the same strain background. We sequenced total RNA and determined transcript abundance.
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| Contributor(s) |
Li Y, Schweizer S, Fromme T, Klingenspor M |
| Citation(s) |
30456392 |
| |
| Submission date |
Sep 12, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Tobias Fromme |
| E-mail(s) |
fromme@tum.de
|
| Organization name |
Technical University of Munich
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| Department |
Molecular Nutritional Medicine
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| Street address |
Gregor-Mendel-Str. 2
|
| City |
Freising |
| ZIP/Postal code |
85354 |
| Country |
Germany |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA490535 |
| SRA |
SRP161606 |
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