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Series GSE119745 Query DataSets for GSE119745
Status Public on Sep 17, 2018
Title Physical basis for long-distance communication along meiotic chromosomes (ChIP-seq)
Organism Schizosaccharomyces pombe
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Viable gamete formation requires segregation of homologous chromosomes connected, in most species, by crossovers. DNA double-strand break (DSB) formation and the resulting crossovers are regulated at multiple levels to prevent overabundance along chromosomes. Meiotic cells coordinate these events between distant sites, but the physical basis of long-distance chromosomal communication has been unknown. We show that DSB hotspots up to ~200 kb (~35 cM) apart form clusters via hotspot-binding proteins Rec25 and Rec27 in fission yeast.  Clustering coincides with hotspot competition and interference over similar distances. Without Tel1 (ATM tumor-suppressor homolog), DSB and crossover interference become negative, reflecting coordinated action along a chromosome. These results indicate that DSB hotspots within a limited chromosomal region and bound by their protein determinants form a clustered structure that, via Tel1, allows only one DSB per region. Such a “roulette” process among clusters explains the observed pattern of crossover interference in fission yeast. Key structural and regulatory components of clusters are phylogenetically conserved, suggesting conservation of this vital regulation. Based on these observations, we discuss variations on a model in which clustering and competition between DSB sites leads to DSB interference and in turn produces crossover interference.
Overall design Eight total samples split between two sequencing runs. These eight samples consist of four pairs of immunoprecipitations. Each pair is the immunopreciptated DNA being ligated before or after crosslinkeds were removed.
Contributor(s) Fowler KR, Hyppa RW, Cromie GA, Smith GR
Citation(s) 30217891
Submission date Sep 10, 2018
Last update date Mar 25, 2019
Contact name Kyle Fowler
Organization name University of California San Francisco
Street address 600 16th Street
City San Francisco
State/province California
ZIP/Postal code 94158
Country USA
Platforms (2)
GPL13988 Illumina HiSeq 2000 (Schizosaccharomyces pombe)
GPL17225 Illumina HiSeq 2500 (Schizosaccharomyces pombe)
Samples (8)
GSM3384164 Rec27-GFP_interacting
GSM3384165 Rec27-GFP_de-crosslinked
GSM3384166 Rec27-GFP_rec8D_interacting
This SubSeries is part of SuperSeries:
GSE119921 Physical basis for long-distance communication along meiotic chromosomes
BioProject PRJNA490355
SRA SRP161503

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119745_RAW.tar 2.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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