|
| Status |
Public on Dec 21, 2018 |
| Title |
Differential gene expression in EpCAM+ pancreatic cancer cells with Lifr deficiency |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study is to investigate the molecular mechanisms of LIFR signaling in pancreatic cancer cells isolated from the classical pancreatic ductal adenocarcinoma mouse model KrasLSL-G12D;Tp53f/f;Rosa26LSL-Luc;Pdx1-Cre mice
EpCAM+ pancreatic cancer cells were isolated by FACS from pancreatic tumors developed in Lifrf/f;KrasLSL-G12D/+;Trp53f/f;Rosa26LSL-Luc;Pdx1-Cre or LifrWT;KrasLSL-G12D/+;Trp53f/f;Rosa26LSL-Luc;Pdx1-Cre mice respectively and directly lysed for RNA extraction
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| Overall design |
Tumors were resected from Lifrf/f;KrasLSL-G12D/+;Trp53f/f;Rosa26LSL-Luc;Pdx1-Cre or LifrWT;KrasLSL-G12D/+;Trp53f/f;Rosa26LSL-Luc;Pdx1-Cre mice at 44~49 days of age
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| |
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| Contributor(s) |
Shi Y, Hunter T |
| Citation(s) |
30996350 |
| |
| Submission date |
Sep 09, 2018 |
| Last update date |
May 26, 2019 |
| Contact name |
Galina Erikson |
| E-mail(s) |
erikson@ie-freiburg.mpg.de
|
| Phone |
+49 (0)761 5108-0
|
| Organization name |
Max Planck Institute of Immunobiology and Epigenetics
|
| Street address |
Stübeweg 51
|
| City |
Freiburg im Breisgau |
| ZIP/Postal code |
79108 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA490027 |
| SRA |
SRP160439 |
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