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| Status |
Public on Dec 18, 2018 |
| Title |
Effects of transcription factor CEBPA knockdown on vitamin D target gene regulation in THP-1 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Three independent repeats of siRNA knockdown of CEBPA, followed by 24h vitamin D treatment, RNA extraction and RNA-seq.
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| Overall design |
THP-1 cells were transfected for 24h with either negative control siRNA or siRNA against transcription factor gene CEBPA, followed by 24h treatment with vitamin D or solvent (EtOH) before performing RNA extraction and RNA-seq.
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| Contributor(s) |
Nurminen V, Neme A, Carlberg C |
| Citation(s) |
30550771, 33753848 |
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| Submission date |
Sep 05, 2018 |
| Last update date |
Apr 06, 2021 |
| Contact name |
Carsten Carlberg |
| E-mail(s) |
carsten.carlberg@uef.fi
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| Organization name |
University of Eastern Finland
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| Department |
Institute of Biomedicine
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| Street address |
Yliopistonranta 1D
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| City |
Kuopio |
| ZIP/Postal code |
70211 |
| Country |
Finland |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (12)
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| GSM3377471 |
THP-1 24h after EtOH treatment, 1st replicate NC1 siRNA |
| GSM3377472 |
THP-1 24h after 1,25D treatment, 1st replicate NC1 siRNA |
| GSM3377473 |
THP-1 24h after EtOH treatment, 1st replicate CEBPA siRNA |
| GSM3377474 |
THP-1 24h after 1,25D treatment, 1st replicate CEBPA siRNA |
| GSM3377475 |
THP-1 24h after EtOH treatment, 2nd replicate NC1 siRNA |
| GSM3377476 |
THP-1 24h after 1,25D treatment, 2nd replicate NC1 siRNA |
| GSM3377477 |
THP-1 24h after EtOH treatment, 2nd replicate CEBPA siRNA |
| GSM3377478 |
THP-1 24h after 1,25D treatment, 2nd replicate CEBPA siRNA |
| GSM3377479 |
THP-1 24h after EtOH treatment, 3rd replicate NC1 siRNA |
| GSM3377480 |
THP-1 24h after 1,25D treatment, 3rd replicate NC1 siRNA |
| GSM3377481 |
THP-1 24h after EtOH treatment, 3rd replicate CEBPA siRNA |
| GSM3377482 |
THP-1 24h after 1,25D treatment, 3rd replicate CEBPA siRNA |
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| This SubSeries is part of SuperSeries: |
| GSE119556 |
The pioneer factor CEBPA modulates vitamin D signaling |
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| Relations |
| BioProject |
PRJNA489500 |
| SRA |
SRP159689 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE119551_abundance.csv.gz |
633.9 Kb |
(ftp)(http) |
CSV |
| GSE119551_differential_expression_CEBPA_V_E.csv.gz |
783.5 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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