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Status |
Public on Aug 31, 2020 |
Title |
Microarray-based miRNA expression in fluid shear stress-stimulated collateral blood vessels |
Platform organisms |
Caenorhabditis elegans; Drosophila melanogaster; Danio rerio; Homo sapiens; Mus musculus; Rattus norvegicus |
Sample organism |
Rattus norvegicus |
Experiment type |
Non-coding RNA profiling by array
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Summary |
collateral tissue from rats was isolated to assess miRNA expression changes between quiescent and growing blood vessels A total of ten total RNA samples were processed. 800 ng of total RNA from each sample was subjected to two different labeling reactions (AF665 and AF546). Two differentially labeld reactions were combined into one tube and loaded onto the arrays.
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Overall design |
Two condition experiment: growing vs quiescent; 5 biological replicates in each group.
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Contributor(s) |
Schierling W, Troidl K |
Citation missing |
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Submission date |
Sep 04, 2018 |
Last update date |
Sep 02, 2020 |
Contact name |
Kerstin Troidl |
E-mail(s) |
kerstin.troidl@mpi-bn.mpg.de
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Organization name |
Max-Planck-Institute for Heart and Lung Research
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Street address |
Ludwigstr. 43
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City |
Bad Nauheim |
ZIP/Postal code |
61231 |
Country |
Germany |
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Platforms (1) |
GPL25527 |
NCode Multi-Species miRNA Microarray V2 |
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Samples (10)
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GSM3374643 |
control rat collateral tissue replicate 1 |
GSM3374644 |
control rat collateral tissue replicate 2 |
GSM3374645 |
control rat collateral tissue replicate 3 |
GSM3374646 |
control rat collateral tissue replicate 4 |
GSM3374647 |
control rat collateral tissue replicate 5 |
GSM3374648 |
shear stress-stimulated rat collateral tissue replicate 1 |
GSM3374649 |
shear stress-stimulated rat collateral tissue replicate 2 |
GSM3374650 |
shear stress-stimulated rat collateral tissue replicate 3 |
GSM3374651 |
shear stress-stimulated rat collateral tissue replicate 4 |
GSM3374652 |
shear stress-stimulated rat collateral tissue replicate 5 |
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Relations |
BioProject |
PRJNA489255 |