|
| Status |
Public on Sep 04, 2018 |
| Title |
Human Adipocytes Regulate Gene Expression in Triple-negative Breast Cancer Assessed by NGS Sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Adipocyte was found play a pivotal role in tumorgenesis, progression and metastasis in breast cancer. However, affection of adipocyte on gene expression profile in triple-negative breast cancer (TNBC) is still not clear. In the present study, firstly we reported the gene expression profiles of TNBC regulated by human adipocytes using NGS. TNBC cell MDA-MB-231 was used in this study. MDA-MB-231 cells were treated with medium derived from adipocytes culture supernatants. Using two different RNA libraries, we sequenced the complete transcriptome(including mRNA, lncRNA, circleRNA and small RNA) of MDA-MB-231 treated with medium derived from adipocytes culture supernatants.
|
| |
|
| Overall design |
NGS sequencing of wild type and adipocyte treated MDA-MB-231 cells, and identifing differentiated expressed genes
|
| |
|
| Contributor(s) |
Tao Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 03, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Zhonghua Tao |
| Organization name |
Fudan University Shanghai Cancer Center
|
| Street address |
No.270, Dong'an Road, Xuhui.
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platforms (2) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA489010 |
| SRA |
SRP159457 |
Less...
More...