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| Status |
Public on Nov 07, 2018 |
| Title |
Comparative transcriptomics of Aspergillus niger when deleted or overexpressed for the putative transcription factors MjkA, MjkB and the histon deactelyase HdaX |
| Organism |
Aspergillus niger |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Expression profiling of two ORFs encoding putative transcription factors: An07g07370 (TF1/MjkA) and An12g07690 (TF2/MjkB), and a histone deacetylase (An09g06520, HdaX) under carbon-limited batch cultivations (biological duplicate runs) in Aspergillus niger.
Methods: Single deletion strains for TF1, TF2 and HD, respectively, (ii) a double deletion strain for TF1 and TF2, and (iii) individual conditional overexpression mutants for TF1, TF2 and HD using the Tet-on system were analysed. Samples were taken at exponential and post-exponential growth phase (technical duplicate) and analysed by RNA-Seq analysis (DOI).
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| |
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| Overall design |
mRNA profiles of MjkA and MjkB transcription factors deletion and over expression strains in A. niger in duplicates, Illumina HiSeq
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| |
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| Contributor(s) |
Kwon MJ |
| Citation(s) |
30496528 |
| |
| Submission date |
Aug 31, 2018 |
| Last update date |
Feb 06, 2019 |
| Contact name |
Paul Schäpe |
| E-mail(s) |
paul.schaepe@tu-berlin.de
|
| Phone |
+493031472271
|
| Organization name |
TU-Berlin
|
| Department |
Applied and Molecular Microbiology
|
| Street address |
Gustav-Meyer-Allee 25
|
| City |
Berlin |
| ZIP/Postal code |
13355 |
| Country |
Germany |
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| Platforms (1) |
| GPL25519 |
Illumina HiSeq 4000 (Aspergillus niger) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA488723 |
| SRA |
SRP159238 |
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