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| Status |
Public on Sep 26, 2018 |
| Title |
Transcriptomes change differerntly in differernt cancer cells upon EPZ-6438 treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) to find out the difference between EZH2 inhibitor treatment and DMSO group in each cancer cell line, and find the relationship between transcriptomes change and drug sensivitity.
Methods: mRNA profiles of cell lines were generated using Illumina PE150, then GSEA was used for cellular pathways analysis
Results: The result showed 53 common oncogenic signatures were enriched in RNA-seq data. For example, KRAS.300_UP.V1_UP, MEK_UP.V1_UP were statistically enriched in the RNA-seq data. In addition, some oncogenic signatures were only enriched in certain cancer cell lines. For example, 101 and 12 signatures from the RNA-seq were statistically enriched in U2932 and SMMC-7721, respectively.
Conclusions: Our study systematically examined the cellular transcriptomes affected by EPZ-6438, with biologic replicates, generated by RNA-seq technology. EZH2 inhibition results in the transcriptional activation of multiple onco-pathways in a cell-context dependent manner, which may underline the resistance to EZH2 inhibition.
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| Overall design |
mRNA profiles of 3 cancer cell lines treated with 1 μM EPZ-6348 or DMSO for 6 days, using NEBNext® UltraTM RNA Library Prep it for Illumina® (NEB, USA).
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| Contributor(s) |
Geng M, Ding J |
| Citation(s) |
30220457 |
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| Submission date |
Aug 27, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Juan Yan |
| E-mail(s) |
yanjuan1357@163.com
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| Phone |
15002135233
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| Organization name |
Shanghai Institute of Materia Medica
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| Department |
Antitumor Pharmacology
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| Lab |
Meiyu Geng
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| Street address |
555 Zuchongzhi Road
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| City |
Shanghai |
| ZIP/Postal code |
201203 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA488071 |
| SRA |
SRP158917 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE119088_S7721_EvsS7721_c_DEG.xls.gz |
2.7 Mb |
(ftp)(http) |
XLS |
| GSE119088_U2932_EvsU2932_c_DEG.xls.gz |
2.7 Mb |
(ftp)(http) |
XLS |
| GSE119088_pfeif_Evspfeif_c_DEG.xls.gz |
2.7 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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