|
| Status |
Public on Nov 06, 2018 |
| Title |
Mapping the differential distribution of the cohesin subunit Scc1 in Trypanosoma brucei brucei. |
| Organism |
Trypanosoma brucei brucei |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Chromatin immunoprecipitation of genomic loci in Trypanosoma brucei where Scc1 is deposited. This was achieved by deletion of one Scc1 allele and N-terminal tagging of the second Scc1 allele with a Ty1-tag. During the ChIP experiment, the DNA was crosslinked and cells were permeabilized with digitonin. DNA was fragmented by sonication with a Covaris instrument. DNA bound to Ty1-Scc1 was pulled down by using a BB2 anti-Ty1 antibody. Cross links are reversed and the DNA was purified and prepared for Illumina sequencing.
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| |
|
| Overall design |
Experiment was done in triplicates, each with an input control. As a control, an input sample was included for each ChIP experiment.
|
| |
|
| Contributor(s) |
Müller LS, NSiegel T |
| Citation(s) |
30333624 |
| |
| Submission date |
Aug 27, 2018 |
| Last update date |
Nov 08, 2018 |
| Contact name |
Konrad U. Förstner |
| E-mail(s) |
foerstner@zbmed.de
|
| Organization name |
ZB MED - Information Centre for Life Sciences
|
| Department |
Information Services
|
| Lab |
Förstner Lab
|
| Street address |
Gleueler Str. 60
|
| City |
Cologne |
| State/province |
North Rhine-Westphalia |
| ZIP/Postal code |
50931 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL22141 |
Illumina NextSeq 500 (Trypanosoma brucei brucei) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA488057 |
| SRA |
SRP158910 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE119071_RAW.tar |
931.3 Mb |
(http)(custom) |
TAR (of WIG) |
| GSE119071_ws1ss1_ratio_L1800344_Scc1_Ty1_wt_soni_Input_0_to_HGAP3_Tb427v9_bwa.sorted.mapq10_vs_L1800343_Scc1_Ty1_wt_soni_ChIP_0_to_HGAP3_Tb427v9_bwa.sorted.mapq10.wig.gz |
198.4 Mb |
(ftp)(http) |
WIG |
| GSE119071_ws1ss1_ratio_L1800469_Scc1_Ty1_wt_soni_Input_1_to_HGAP3_Tb427v9_bwa.sorted.mapq10_vs_L1800468_Scc1_Ty1_wt_soni_ChIP_1_to_HGAP3_Tb427v9_bwa.sorted.mapq10.wig.gz |
164.3 Mb |
(ftp)(http) |
WIG |
| GSE119071_ws1ss1_ratio_L1800475_Scc1_Ty1_wt_soni_Input_2_to_HGAP3_Tb427v9_bwa.sorted.mapq10_vs_L1800474_Scc1_Ty1_wt_soni_ChIP_2_to_HGAP3_Tb427v9_bwa.sorted.mapq10.wig.gz |
167.2 Mb |
(ftp)(http) |
WIG |
| GSE119071_ws2001ss501_ratio_L1800344_Scc1_Ty1_wt_soni_Input_0_to_HGAP3_Tb427v9_bwa.sorted.mapq10_vs_L1800343_Scc1_Ty1_wt_soni_ChIP_0_to_HGAP3_Tb427v9_bwa.sorted.mapq10.wig.gz |
1.1 Mb |
(ftp)(http) |
WIG |
| GSE119071_ws2001ss501_ratio_L1800469_Scc1_Ty1_wt_soni_Input_1_to_HGAP3_Tb427v9_bwa.sorted.mapq10_vs_L1800468_Scc1_Ty1_wt_soni_ChIP_1_to_HGAP3_Tb427v9_bwa.sorted.mapq10.wig.gz |
1.1 Mb |
(ftp)(http) |
WIG |
| GSE119071_ws2001ss501_ratio_L1800475_Scc1_Ty1_wt_soni_Input_2_to_HGAP3_Tb427v9_bwa.sorted.mapq10_vs_L1800474_Scc1_Ty1_wt_soni_ChIP_2_to_HGAP3_Tb427v9_bwa.sorted.mapq10.wig.gz |
1.1 Mb |
(ftp)(http) |
WIG |
| GSE119071_ws501ss101_ratio_L1800344_Scc1_Ty1_wt_soni_Input_0_to_HGAP3_Tb427v9_bwa.sorted.mapq10_vs_L1800343_Scc1_Ty1_wt_soni_ChIP_0_to_HGAP3_Tb427v9_bwa.sorted.mapq10.wig.gz |
5.3 Mb |
(ftp)(http) |
WIG |
| GSE119071_ws501ss101_ratio_L1800469_Scc1_Ty1_wt_soni_Input_1_to_HGAP3_Tb427v9_bwa.sorted.mapq10_vs_L1800468_Scc1_Ty1_wt_soni_ChIP_1_to_HGAP3_Tb427v9_bwa.sorted.mapq10.wig.gz |
5.2 Mb |
(ftp)(http) |
WIG |
| GSE119071_ws501ss101_ratio_L1800475_Scc1_Ty1_wt_soni_Input_2_to_HGAP3_Tb427v9_bwa.sorted.mapq10_vs_L1800474_Scc1_Ty1_wt_soni_ChIP_2_to_HGAP3_Tb427v9_bwa.sorted.mapq10.wig.gz |
5.3 Mb |
(ftp)(http) |
WIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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