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| Status |
Public on May 14, 2020 |
| Title |
Intrinsic toxicity of LCDD monoclonal Ig free light chain on plasma cells (PC) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We analyzed the transcriptome (RNA-seq) of mouse plasma cells (PC) expressing a human Ig light chain from a patient suffering light chain deposition disease (LCDD) as compared to control plasma cells (WT and DH-LMP2A, the latter producing no complete immunoglobulins, only free Ig light chains).
Ig light chains causing LCDD present structural peculiarities leading to their aggregation and deposition into tissues and organs, mainly the kidney. We sought to determine if plasma cells producing these abnormal Ig could present specific phenotypes.
The aim of the present study is to analyse the transcriptomic signature of plasma cells producing abnormal Ig free light chains.
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| Overall design |
Spleen plasma cells from non-immunized mice were magnetically-purified using CD138 beads (purity > 80%). Each sample corresponds to the total CD138+ plasma cells from one spleen. Cells are from 3-month-old mice.
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| Contributor(s) |
Paquet A, Magnone V, Sirac C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 26, 2018 |
| Last update date |
May 14, 2020 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
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| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
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| Street address |
660 route des lucioles
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| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE119050 |
Toxicity of LCDD monoclonal Ig free light chains |
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| Relations |
| BioProject |
PRJNA487941 |
| SRA |
SRP158844 |
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