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| Status |
Public on Mar 01, 2019 |
| Title |
Prostaglandin E2 inhibits pro-fibrotic function of human pulmonary fibroblasts by disrupting Ca2+-signaling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Identification of gene expression in human lung fibroblasts, including analysis of impact of IPF (Idiopathic Pulmonary Fibrosis) as well as TGFβ stimulation, with particular emphasis on prostanoid receptors and related pathways.
Methods: Truseq stranded mRNA sequencing, via Illumina Hiseq 4000. Single reads, 75bp, ~25 million reads per sample. Quantification of gene expression in TPM using Kallisto, followed by Tximport. mRNA isolated via Qiagen RNeasy kit, with on-column DNase1 digestion.
Results: Quantification of gene expression in primary human lung fibroblasts, including quantification of prostanoid GPCRs.
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| Overall design |
Transcriptomes of human lung fibroblasts (HPFs), assayed via RNA-seq for HPFs with/wo TGFβ treatment, from normal donors and patients with IPF; n =3 for each group, with 4 groups.
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| Contributor(s) |
Sriram K, Insel P, Janssen L, Mukherjee S |
| Citation(s) |
30758990 |
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| Submission date |
Aug 24, 2018 |
| Last update date |
Feb 18, 2022 |
| Contact name |
Paul A Insel |
| Phone |
858 534 2298
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| Organization name |
UC San Diego
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| Department |
Pharmacology
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| Lab |
Insel
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| Street address |
9500 Gilman Drive
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA487726 |
| SRA |
SRP158749 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE119007_Table_S1_Gene_expression_in_CPM.xlsx |
2.5 Mb |
(ftp)(http) |
XLSX |
| GSE119007_Table_S1_Gene_expression_in_TPM_withGsGPCRexpression.xlsx |
13.1 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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