|
| Status |
Public on Jun 26, 2008 |
| Title |
Lim Homeobox Gene, LHX8, Is Essential for Mouse Oocyte Differentiation and Survival |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Lhx8 is a member of the LIM-homeobox transcription factor family and preferentially expressed in oocytes and germ cells within the mouse ovary. We discovered that Lhx8 knockout females lose oocytes within 7 days after birth. At the time of birth, histological examination shows that Lhx8 deficient (Lhx8(-/-)) ovaries are grossly similar to the newborn wild type ovaries. Lhx8(-/-) ovaries fail to maintain the primordial follicles and the transition from primordial to growing follicles does not occur. Lhx8(-/-) ovaries misexpress oocyte-specific genes such as Gdf9, Pou5f1, and Nobox. Very rapid loss of oocytes may partly be due to drastic the down-regulation of Kit and Kitl in Lhx8(-/-) ovaries. We compared Lhx8(-/-) and wild-type ovaries using Affymetrix 430 2.0 microarray platform. Eighty (44%) of 180 of the genes down-regulated more than 5-fold in Lhx8(-/-) ovaries were preferentially expressed in oocytes, whereas only 3 (2%) of 146 genes up-regulated more than 5-fold in the absence of Lhx8 were preferentially expressed in oocytes. In addition, the comparison of genes regulated in Lhx8(-/-) and Nobox(-/-) newborn ovaries discovered a common set of 34 genes whose expression level is affected in both Lhx8 and Nobox deficient mice. Our findings show that Lhx8 is a critical factor for maintenance and differentiation of the oocyte during early oogenesis and it acts in part by down-regulating the Nobox pathway.
This SuperSeries is composed of the SubSeries listed below.
|
| |
|
| Overall design |
Refer to individual Series
|
| |
|
| Citation(s) |
18509161 |
| |
| Submission date |
Jun 26, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Aleksandar Rajkovic |
| E-mail(s) |
rajkovic@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
| GPL340 |
[MOE430B] Affymetrix Mouse Expression 430B Array |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (11)
|
|
| This SuperSeries is composed of the following SubSeries: |
| GSE7774 |
Transcriptional changes in Lhx8 Null newborn mouse ovaries |
| GSE7775 |
Microarray Analyses of Newborn Mouse Ovaries Lacking Nobox |
| GSE7776 |
Ovarian Transcript Expression in Newborn Mouse |
|
| Relations |
| BioProject |
PRJNA105729 |
Less...
More...