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| Status |
Public on Aug 01, 2021 |
| Title |
Comparison of periosteal, adipose, and dermal CD146+ human pericytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human pericytes demonstrate multilineage differentiation potential, and their descendants participate in tissue homeostasis and repair. Increasing evidence from developmental biology and tissue engineering suggest that regional specification by tissue of origin exists among human pericytes. Here, we sought to define the differentiation of CD146+ human pericytes from skeletal and soft tissue sources. Uncultured CD146+CD31-CD45- pericytes were derived by fluorescent activated cell sorting from human periosteum, adipose, or dermal tissue. Periosteal CD146+CD31-CD45- cells retained canonical features of pericytes, including cell surface marker expression, multilineage differentiation potential, and paracrine induced tubulogenesis. Periosteal pericytes demonstrated a striking tendency to undergo osteoblastogenesis, while soft tissue pericytes did not in vitro or in vivo. Microarray analysis demonstrated substantive differences between periosteal pericytes in comparison to their soft tissue pericyte counterparts. In sum, skeletal and soft tissue pericytes differ in their relative lineage differentiation potential and ability to form bone.
Human tissues were microdissected, digested, and FACS sorted to derived a CD146+CD31-CD45- pericyte population, and cells were expanded in standard growth medium (DMEM, 10% FBS, 1% pen/strep) and nucleic acid isolated at subconfluency
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| Overall design |
Human periosteum, subcutaneous adipose tissue, or dermis was obtained from human tissues (n=3 biologic replicates per site, 9 total), enzymatically digested, and a CD146+CD31-CD45- pericyte population was purified by fluorescent activated cell sorting. Cells were expanded at 37°C in a humidified atmosphere containing 95% air and 5% CO2. All cells were cultured in DMEM medium with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Medium was changed every three d, and nucleic acid was harvested at subconfluency.
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| Contributor(s) |
James AW, Xu J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 23, 2018 |
| Last update date |
Aug 02, 2021 |
| Contact name |
Aaron W James, MD, PhD |
| Organization name |
Johns Hopkins University School of Medicine
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| Department |
Pathology
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| Lab |
Ross 524
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| Street address |
600 North Wolfe Street
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
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| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA487541 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE118962_RAW.tar |
222.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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