|
| Status |
Public on May 01, 2020 |
| Title |
A Functional Role for REST Co-Repressor 2 (Rcor2) in Callosal Development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Pallial functional organization is mediated by the dynamic interplay of a broad spectrum of cellular and molecular cues that promote midline patterning, neurogenesis, axon guidance and neural connectivity. The regulators that orchestrate these seminal processes are still poorly understood. By employing a pallial conditional ablation model, we report that the transcriptional and epigenetic modulator, REST corepressor 2 (Rcor2) plays key roles in organizing the developing corticoseptal boundary. ChIP-seq and RNA-seq analyses reveal that Rcor2 modulates a diverse repertoire of classes of genes with essential roles in CC development, including pallial/subpallial patterning, cell fate specification and axon guidance.
|
| |
|
| Overall design |
ChIP-sequencing performed on embryonic day 14.5 midline cortex of C57/Bl6 strain mice. Immunoprecipitation was carried out using an Rcor2 specific antibody, or a rabbit IgG non specific mock antibody. 2 replicates of each immunoprecipitation were multiplexed using the NEBnext II library prep kit and sequenced through Cornell epigenomcs core facility using a Hiseq2500 platform. RNA-seq was performed on E14.5 cortical midline comparing Rcor2f/f used as controls versus Rcor2f/f/EMX1-iCRE conditional knockouts. 2 Replicates per genotype were used. Multiplexed libraries were generated with the TRUseq Total RNA library prep kit, and sequenced on a Hiseq2500 platform by the Cornell epigenomics core facility.
|
| |
|
| Contributor(s) |
DeJesus CD, Molero AE, Wang P, Zheng D, Gokhan S, Mehler MF |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 20, 2018 |
| Last update date |
May 01, 2020 |
| Contact name |
Mark F Mehler |
| E-mail(s) |
mark.mehler@einstein.yu.edu
|
| Phone |
718-430-4229
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Neuroscience
|
| Lab |
Mehler
|
| Street address |
1300 Morris Park Ave.
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA486810 |
| SRA |
SRP158427 |
Less...
More...