|
| Status |
Public on Aug 01, 2019 |
| Title |
Gene expression profile of human iPSC-derived nephron progenitor cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
During development, nephron progenitor forming one million nephrons, a functional unit in the kidney. However, nephron progenitor ceases before birth in human when they terminally differentiated to the nephron. Our lab established the method for induction of nephron progenitors from mouse Embryonic Stem (ES) cells and/or human induced Pluripotent Stem Cells (iPSCs) (Taguchi et al., Cell Stem Cell. 2014, 2017). For application of induced nephron progenitors to regenerative medicine, a large number of cells are required such as disease modeling and drug screening. To selectively propagate human iPS-derived nephron progenitors in vitro in an undifferentiated state, we developed SIX2-GFP iPS line and optimized culture condition of induced nephron progenitors by modifying our previously developed condition (Tanigawa et al., Cell Rep. 2016). To understand how whole gene expression profiles of human iPS-derived nephron progenitor cells are changed during culture, we isolated nephron progenitor cells by FACS and cultured in our defined culture condition. Purified RNAs from cultured cells at day 7 or un-cultured nephron progenitor cells were analyzed by RNA-seq.
|
| |
|
| Overall design |
In order to propagate the human iPS-derived nephron progenitors, we generated iPSCs that express GFP in the SIX2 locus by TALEN-mediated homologous recombination and then purified SIX2-positive population from iPSCs-derived tissues by FACS. Isolated ITGA8+/PDGFRA-/SIX2-GFP+ cells were cultured for 7 days in our defined conditions and compared uncultured cells.
|
| |
|
| Contributor(s) |
Tanigawa S, Nishinakamura R |
| Citation(s) |
31378669 |
| |
| Submission date |
Aug 18, 2018 |
| Last update date |
Sep 03, 2019 |
| Contact name |
Shunsuke Tanigawa |
| E-mail(s) |
shunsuke_tanigawa@hotmail.com
|
| Phone |
0963736617
|
| Organization name |
Kumamoto University
|
| Department |
Institute of Molecular Embryology and Genetics
|
| Lab |
Kidney development
|
| Street address |
2-2-1 Chuou-ku honjyo
|
| City |
Kuamomoto |
| State/province |
Kumamoto |
| ZIP/Postal code |
8600811 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA486586 |
| SRA |
SRP158308 |
Less...
More...