|
| Status |
Public on Oct 10, 2019 |
| Title |
Genetic interaction analysis of RSA1 deletion strains (GIM screen) |
| Platform organism |
Saccharomyces cerevisiae S288C |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
A S. cerevisiae strain with deletion of RSA1 gene was combined with two collections of yeast mutants: one in which non-essential genes were deleted and one in which an long 3' UTR extension has been added to the mRNA of essential genes (GIM method, Decourty et al., 2008). Combined double-mutant deletion cells growth was quantified using barcodes that are specific for each gene mutation. A reference population was obtained by mixing the results of 15 GIM screens DNA prior to barcode amplification and labeling (Decourty et al., in preparation).
The identification of genetic interactions between deletion of RSA1 and deletion of RTT106, a histone chaperone, indicate a potential role of Rtt106 in snoRNP formation.
|
| |
|
| Overall design |
Two independent GIM experiments were analyzed using two Agilent microarrays
|
| |
|
| Contributor(s) |
Saveanu C |
| Citation(s) |
33767140 |
| |
| Submission date |
Aug 14, 2018 |
| Last update date |
Apr 20, 2021 |
| Contact name |
Cosmin Saveanu |
| Organization name |
Institut Pasteur
|
| Department |
Genomes and Genetics
|
| Lab |
Genetique des Interactions Macromoleculaires
|
| Street address |
25-28 rue du docteur Roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL18088 |
Agilent-026035 Scer_barcode_v2_200911 |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA485996 |
