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| Status |
Public on Aug 10, 2021 |
| Title |
Identification of LIN-29 target genes |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To further explore the regulatory network coordinated by LIN-29, and to possibly uncover potential new roles for this transcription factor, we searched for its downstream target genes at the genomic level. To do this, we chose not to modify LIN-29 expression via loss-of-function mutations in upstream heterochronic pathway components, but rather to temporally misexpress LIN-29 directly and examine changes in development and in gene expression.
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| Overall design |
We carried out early overexpression of LIN-29 by heat-shock inducing hs::lin-29 and hs::control strains in early L3 stage larva, and assessed gene expression by RNA-seq.
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| Contributor(s) |
Abete-Luzi P, Fukushige T, Yun S, Krause MW, Eisenmann DM |
| Citation(s) |
31974205 |
| |
| Submission date |
Aug 10, 2018 |
| Last update date |
Jan 26, 2022 |
| Contact name |
Sijung Yun |
| Organization name |
NIH/JHU
|
| Street address |
8908 Ewing Drive
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20817 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA485538 |
| SRA |
SRP157115 |
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