|
| Status |
Public on Aug 01, 2019 |
| Title |
Unr is a potential post-transcriptional master regulator of protein expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Ribonucleoprotein immunoprecipitation followed by mass spectrometry and RNA-Seq was used to investigate the ‘Upstream of N-RAS’ (UNR) protein interactome across three cancer cell lines. A number of novel UNR-interacting proteins and transcripts were discovered, including the ubiquitin E3 ligase, HUWE1; the nucleolar protein, NARR; the multifunctional protein, SQSTM1; and the erythropoiesis-related protein, LDB1 . GO-term analysis of putative UNR interactors suggested that UNR interacts with both proteins and mRNAs encoding proteins that are involved in RNA binding
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| |
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| Overall design |
Examination of UNR binding to RNA by RIP-RNA-Seq using three replicates of three cell types,
|
| |
|
| Contributor(s) |
Ó Catnaigh P, Ray S, Dyer NP, Leppard K, Ott S, Anderson E |
| Citation missing |
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| |
| Submission date |
Aug 08, 2018 |
| Last update date |
Aug 01, 2019 |
| Contact name |
Nigel Paul Dyer |
| E-mail(s) |
nigel.dyer@warwick.ac.uk
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| Organization name |
University of Warwick
|
| Street address |
Gibbet Hill Road
|
| City |
Coventry |
| ZIP/Postal code |
CV4 7AL |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (36)
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| Relations |
| BioProject |
PRJNA485061 |
| SRA |
SRP156703 |
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