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| Status |
Public on Aug 02, 2018 |
| Title |
Erythro-myeloid progenitors contribute endothelial cells to developing blood vessels |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The earliest blood vessels in the mammalian embryo are formed when endothelial cells (ECs) differentiate from angioblasts and coalesce into tubular networks. Thereafter, the en-dothelium is thought to expand solely by proliferation of pre-existing ECs. Here we show that the earliest precursors of erythrocytes, megakaryocytes and macrophages, the yolk sac-derived erythro-myeloid progenitors (EMPs), provide a complementary source of ECs that are recruited into pre-existing vasculature. Whereas a first wave of yolk sac-resident EMPs contributes ECs to the yolk sac endothelium, a second wave of EMPs colonises the embryo and contributes ECs to intraembryonic endothelium in multiple organs, where they persist into adulthood. By demonstrating that EMPs constitute a hitherto unrecognised source of ECs, we reveal that embryonic blood vascular endothelium expands in a dual mechanism that involves both the proliferation of pre-existing ECs and the incorporation of ECs derived from hematopoietic precursors.
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| Overall design |
RNAseq analysis of FACS-isolated tdTom+ and tdTom- ECs from E12.5 Csf1r-iCre;RosatdTom embryos.
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| Contributor(s) |
Fantin A, Plein A, Ruhrberg C |
| Citation(s) |
30258231 |
| |
| Submission date |
Aug 01, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Alessandro Fantin |
| E-mail(s) |
a.fantin@ucl.ac.uk
|
| Phone |
2076084018
|
| Organization name |
University College London
|
| Department |
UCL Institute of Ophthalmology
|
| Street address |
11-43 Bath Street
|
| City |
London |
| State/province |
- None - |
| ZIP/Postal code |
EC1V 9EL |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA483887 |
| SRA |
SRP155955 |
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