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| Status |
Public on Aug 31, 2018 |
| Title |
Developmentally regulated isoform of HNF4α reprograms the adult liver |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hepatocyte Nuclear Factor 4α (HNF4α), master regulator of hepatocyte differentiation, is regulated by two promoters (P1 and P2). P1-HNF4α but not P2-HNF4α is expressed in normal adult liver while both P1- and P2-HNF4α are expressed in fetal liver and liver cancer. To determine the physiological function of the HNF4α isoforms, we compared P2-HNF4α-expressing exon swap mice to wildtype (WT) using RNA-seq, ChIP-seq, proteomics, protein binding microarrays (PBMs) and metabolomics. P2-HNF4α orchestrates a distinct transcriptomic and metabolomic profile.
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| Overall design |
Liver RNA-seq samples from both genotypes (WT and a7HMZ, P1- and P2-HNF4α respectively) were taken at three time-points as well as a 12-hr fast with both genotypes at the earliest time-point.
Please note that three different time points are indicated in the sample titles as the time of the day, in 24hr notation (i.e. 1030 = 10:30am, 1330 = 1:30pm, and 2030 = 8:30pm).
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| Contributor(s) |
Deans JR, Vuong LM, Sladek FM |
| Citation missing |
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| |
| Submission date |
Aug 01, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Jonathan Robert Deans |
| E-mail(s) |
jdean007@ucr.edu
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| Organization name |
University of California Riverside
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| Department |
Molecular, Cell and Systems Biology
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| Lab |
Sladek Lab
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| Street address |
3111 BSB, University of California Riverside
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| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA483880 |
| SRA |
SRP155943 |
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